ENZYMATIC STUDIES ON THE EFFECT OF ANTIBIOTICS ON THIAMINE METABOLISM : (II) THE PHOSPHORYLATION ACTIVITY OF THIAMINE IN THE LIVER AND BRAIN OF THE RAT WITH BIOAUTOGRAPHY USING ESCHERICHIA COLI MUTANTS

Abstract

Thiaminokinase from rat liver was purified about 5 folds compared with liver supernatant by Mano's method. It was found that esterfied thiamine product derived from thiamine was thiamine diphosphate with bioautography using Escherichia coli mutants. It was found that the activity was not present in the brain of the rat and cat.