抄録
In the preceding paper the production of DBCC was reported by cultivation of Propionibacterium shermanii in a medium containing hydroxocobalamin or cyanocobalamin as a precursor. This paper deals with the production of DBCC by cell suspension of the bacterium. Under the exeprimental condition almost complete conversion of hydroxocobalamin to the coenzyme was achieved in a concentration as high as 50μg/ml, which was about 10 times higher than that of the growing cell method. As the concentration of the precursor increased moreover, the degree of the transformation decreased and the formation of an unidentified vitamin B_<12> analogue was observed. The effectiveness of cyanocobalamin as the precursor was only one third of that of hydroxocobalamin in this method.
