抄録
When TATD is incubated with washed blood cells, a detectable amount of free thiamine has been noted in the surrounding fluid in addition to intracellular accumulation of thiamine. Mechanism of the appearance of the extracellular thiamine was investigated. Results showed that the factor(s) which was formed from the reaction of TATD with the blood cells contributed to the reduction of residual TATD in surrounding medium. The factor(s) appears to be the reduced form of the lipoate moiety (or its catabolite) liberated from TATD by the cells. Reduction of TATD and iso-TATD by washed blood cells was inhibited by SH-blocking agents. No considerable effect is observed by using KCN and 2,4-dinitrophenol. Whereas arsenic acid did not affect the TATD reduction by glutathione, the thiamine liberation from TATD by blood cells was depressed effectively by this reagent. This suggests the concerning of vicinal dithiol as well as monothiol in the TATD reduction by the blood cells.
