抄録
Thiamine and its mono-, di- and triphosphates were successfully separated on thin layer of Silica gel G, using pyridine・water・ammonia・methanol・glacial acetic acid (6 : 6 : 5 : 1 : 1) as developer. The Rf values of thiamine and its mono-, di- and triphosphates were found to be 0.92,0.50,0.17 and 0.08,respectively. The detection of spots was made by thiochrome fluolescence method. For this purpose the mixture of ethanol・10% NaOH・2.5%K-ferricyanide (2 : 1 : 0.05) was found to be most suitable.
