Sorbyl-CoA formation method (Wakil and Hubscher, 1960) was quite specific for determination of CoA, and neither panthetheine, phosphopantetheine nor dephospho-CoA disturbed its determination. The amount of pantetheine in normal rat liver was much smaller than those of both free pantothenic acid and CoA. It was recognized that the content of dephospho-CoA plus phophopantetheine is next to that of CoA in rat liver by combined using the sorbyl-CoA method and the microbiological assay of pantetheine after treatment with intestinal phosphatase. The normal distribution pattern of the various intermediates was described.
