Abstract
Thiamine was easily adsorbed by cells of Sacch. cerevisiae in Reader's medium, when cells had been harvested at the exponential phase, but it was poorly adsorbed by cells at the later stationary phase. When the cells at the stationary phase had been incubated for growth in a fresh medium, the cells harvested, were demonstrated to be restored to adsorb thiamine strongly. The extraordinary accumulation of thiamine in yeast cells was shown to be correlated to their status of harvesting rate of growth, number of budding cells and higher contents of nucleic acids in cells, as shown in Fig. 3 and Table 3. It was suggested that the thiamine accumulation in yeast cells depended upon the active physiological state of the cells prior to the cell division.
