抄録
O-Benzoylthiamine(OBT), when incubated with thiamine-poor L. fermenti for 20 minutes, was proved to be not only poorly absorbable by the cells but also strongly inhibitory to uptake of thiamine by the cells in the broth containing thiamine. Inhibition of thiamine-uptake on Lactobacilli at the presence of OBT was clearly shown to be competitive but thiamine-uptake on Lactobacilli, when previously incubated with OBT and washed by saline at the absence of OBT, was proved to be equal to that on fresh Lactobacilli. Thus competition between thiamine and OBT was observed in the broth, not in the cells. The growth of L. fermenti in the broth containing thiamine and OBT ; 1 : 5 was shown to be equall to that without OBT but thiamihe contents of the cells during the growth was proved to be lower than those without OBT. The growth of L. fermenti in the broth containing" thiamine and OBT; 1 : 200(or 400) was retarded as compared with that without OBT and thiamine contents of the cells especially at the lag period were significantly lower than the control.
