Abstract
In the biosynthesis of thiamine from pyrimidine (I) and thiazole moieties by Sacch. cerevisiae, desthiothiamine (II) was efficiently utilized in the place of (I) but the diazepine compound (III) was not utilized unless (III) was hydrolysed by 10% HCl. When thiamine in the alkaline solution was incubated with or without glycine for hours, (II) and (III) in the reaction mixture were estimated by thiamine-biosynthesis method after their separation through paper partition chromatography. It was demonstrated that at the presence of glycine (II) was greatly increased but at its absence (III) was predominant, although (II) and (III) was always detected in the reaction mixtures with or without glycine. Formation of (II) was more or less accelerated at the presence of other amino acids except aromatic or heterocyclic amino acids but the highest yield of (II) was observed by addition of glycine.
