Abstract
Thiamine production by growing cells of a temperature-sensitive thiazoleless mutant derived from E. coli K12,KG 801,was about a half that of its parent. Glycine, which was found to be able to replace thiazole for the growth of this mutant, stimulated the production of thiamine in the presence of a excess of hydroxymethylpyrimidine by washed cell suspension of the mutant, which is presumed to depend on the synthesis of thiazole in the mutant. A possibility of glycine as precursor in the biosynthesis of thiazole in E. coli was discussed.
