ゲルろ過による脂溶性ビタミンの分離 : (V)製剤中のビタミンDの定量
1970 年 42 巻 2 号 p. 107-111
詳細
1970 年 42 巻 2 号 p. 107-111
The determination of vitamin D in pharmaceutical preparations by the method described in Vitamins 42,99 (1970) was examined. Hydrolysis of the sample was carried out in ethanolic potassium hydroxide by addition of antioxidant such as mixture of hydroquinone and pyrogallol. The unsaponifiable fraction was fractionated on Sephadex LH-20 column as the gel bed and chloroform as the eluting solvent. The eluted vitamin D was purified by chromatography on the weakened Florex XXS column. The antimony trichloride reagent of Nield with acetic anhydride color inhibitor and the two wave-length reading procedure provided corrected values of vitamin D in the presence of interfering substances. Analytical results of the method applied to a series of pharmaceutical products were almost satisfactory.
