抄録
The growth-inhibition of the yeast cells in a logarithmic phase of growth by several kinds of detergents of quarternary ammonium type has been investigated. In all the detergents tested, the concentrations of detergents inhibiting by 50% the protein synthesis and the multiplication of viable cells were considerably lower than those inhibiting by 50% the increase of turbidity. The inhibition of the detergents was significantly restored by either cell-washings or simple dilution of cultures. When ammonium salts were used as sole nitrogen source, there was progressively lesser growth inhibition by lauryl-dimethyl-benzylammonium chloride (L-B_Z) on increasing concentrations of ammonium sulfate, while the increasing concentrations of ammonium acetate stimulated inversely the inhibition. Addition of nicotinic acid led to considerable reversal against the inhibition by L-B_Z, but none of nicotinamide and NAD exhibited the effect.
