Abstract
The urinary excretion and metabolites of the benzoic acid moiety of O, S-dibenzoyl thiamine (DBT) were studied in normal, CCl_4-injured or choline-deficient rats after oral administration of the compound labeled at 1-[^<14>C]-benzoic acid of DBT. In the normal rats, 44〜67,62〜85 and 86〜89% of the ingested radioactivity were excreted into the urine during the first 3,6 and 24 hours, respectively, after the administration. Of the urinary radioactivity, about 95% was identified as hippuric acid by means of thin layer chromatography and ion exchange column chromatography. Neither O-benzoylthiamine nor DBT could be detected in the urine as radioactive compounds. These results indicated that the benzoic acid moiety of orally administered DBT was almost quantitatively excreted into the urine as hippuric acid. These metabolic profiles in the normal rats were hardly affected in rats bearing CCl_4-injured liver or fatty liver induced by choline deficiency.
