VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
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Volume 50, Issue 11
Displaying 1-5 of 5 articles from this issue
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  • Biosynthesis of the Isoalloxazine Ring : Studies on the Purine, Nucleotide Precursor of the Biosynthesis
    Kenji NAKAJIMA
    Article type: Article
    1976 Volume 50 Issue 11 Pages 393-407
    Published: November 25, 1976
    Released on J-STAGE: March 06, 2018
    DOIhttps://doi.org/10.20632/vso.50.11_393
    JOURNAL FREE ACCESS
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  • The Release of L-Ascorbic acid and L-Dehydroascorbic acid from Human Erythrocyte
    Junya TERUUCHI, Masato OKAMURA
    Article type: Article
    1976 Volume 50 Issue 11 Pages 409-413
    Published: November 25, 1976
    Released on J-STAGE: March 06, 2018
    DOIhttps://doi.org/10.20632/vso.50.11_409
    JOURNAL FREE ACCESS
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    The release of L-ascorbic acid (AsA) and L-dehydroascorbic acid (DAsA) from human erythrocyte was investigated in vitro. The erythrocyte, preincubated with DAsA, was used. It contained DAsA and AsA produced by reduction of DAsA in erythrocyte. AsA was released very slowly into the medium from the erythrocyte to an equilibrium state during 20 hours. The release of DAsA was rapidly and its concentration in the medium became equal to that in the cell within one minute.
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  • Metabolic Fate of the Benzoic acid Moiety of O, S-Dibenzoyl thiamine in Rats
    Fumio TOMINAGA, Kouji FURUNO, Kozo SHIMAKAWA, Ziro SUZUOKI
    Article type: Article
    1976 Volume 50 Issue 11 Pages 415-420
    Published: November 25, 1976
    Released on J-STAGE: March 06, 2018
    DOIhttps://doi.org/10.20632/vso.50.11_415
    JOURNAL FREE ACCESS
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    The urinary excretion and metabolites of the benzoic acid moiety of O, S-dibenzoyl thiamine (DBT) were studied in normal, CCl_4-injured or choline-deficient rats after oral administration of the compound labeled at 1-[^<14>C]-benzoic acid of DBT. In the normal rats, 44〜67,62〜85 and 86〜89% of the ingested radioactivity were excreted into the urine during the first 3,6 and 24 hours, respectively, after the administration. Of the urinary radioactivity, about 95% was identified as hippuric acid by means of thin layer chromatography and ion exchange column chromatography. Neither O-benzoylthiamine nor DBT could be detected in the urine as radioactive compounds. These results indicated that the benzoic acid moiety of orally administered DBT was almost quantitatively excreted into the urine as hippuric acid. These metabolic profiles in the normal rats were hardly affected in rats bearing CCl_4-injured liver or fatty liver induced by choline deficiency.
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  • Gas-Liquid Chromatographic Determination of Vitamin D_2 and Ergosterol in Shiitake (Lentinus edodes)
    Tadashi KOBAYASHI, Atsuko ADACHI, keiko FURUTA
    Article type: Article
    1976 Volume 50 Issue 11 Pages 421-429
    Published: November 25, 1976
    Released on J-STAGE: March 06, 2018
    DOIhttps://doi.org/10.20632/vso.50.11_421
    JOURNAL FREE ACCESS
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    Gas-liquid chromatographic (GLC) determination of vitamin D_2 and ergosterol in Shiitake (Lentinus edodes) was investigated and simple routine methods were established. The method for determination of vitamin D_2 includes alkali-saponification, isolation of the unsaponifiable matters, thin-layer chromatography (TLC), trimethylsilylation and GLC. When contents of vitamin D_2 are very small and comparatively large amounts of sterols exist in a sample, however, digitonin-Celite column chromatography should be added before TLC in order to eliminate the influences of sterols. On the other hands, ergosterol is determined by applying directly the trimethylsilyl ethers of the unsaponifiable matters to GLC. When dry Shiitake were irradiated by the ultraviolet lights of 3 E-Viton/cm^2 (30 μW/cm^2), the relationship between yield of vitamin D_2 and irradiation time was proportional and the yield of vitamin D_2 was 29IU/min. The estimated values of ergosterol in commercial dry Shiitake obtained from different production places were distributed in the range of 0.25〜0.74%, while that in a commercial raw sample was 0.05%.
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  • Specific Adsorption of Pyridoxal 5'-phosphate to Aminoethyl-Sephadex and Its Application to Purification of Pyridoxal 5'-phosphate
    Ryo-hei YAMADA, Takahiko TSUJI
    Article type: Article
    1976 Volume 50 Issue 11 Pages 431-435
    Published: November 25, 1976
    Released on J-STAGE: March 06, 2018
    DOIhttps://doi.org/10.20632/vso.50.11_431
    JOURNAL FREE ACCESS
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    Aminoethyl derivative of Sephadex G-25 specifically adsorbed pyridoxal 5'-phosphate (PALP) among the six vitamin B_6 compounds under appropriate conditions. Optimum pH for the adsorption was around 9,and elution was effected by the use of acids, amino compounds or buffers of high ionic strength. The coloring of PALP adsorbed besides above facts indicated that the adsorption was mainly due to Schiff base formation between PALP and the aminoethyl side chain of Sephadex and also due to anion exchanger function of the same side chain. Isolation of PALP produced by incubation of pyridoxine with Escherichia coli cells was attempted by the use of aminoethyl-Sephadex columns, and a spectrophotometrically pure preparation of PALP was readily obtained.
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