ビタミン
Online ISSN : 2424-080X
Print ISSN : 0006-386X
微生物学的定量法を併用したミクロ高速液体クロマトグラフィーによるコバラミン類縁体の分離と定量
三谷 璋子佐藤 一精
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1984 年 58 巻 4 号 p. 163-167

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In the separation of cobalamins by micro-high-performance liquid chromatography (micro-HPLC), each cobalamin was detected first by UV absorption and then by microbiological method in order to improve the sensitivity of the system. For the elution of cobalamins, methanol concentration in 0.05 M sodium acetate buffer, pH 4.0 as the mobile phase was changed stepwise and cobalamins were eluted in the order of OH-B_<12>, HSO_3-B_<12>, CN-B_<12>, AdoB_<12> and CH_3-B_<12>. By this micro-HPLC method combined with microbiological determination, the sensitivity increased about a hundred times in comparison with that already reported with regard to HPLC.
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© 1984 日本ビタミン学会

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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