Measurement of Vitamin K_3 in Foods by High-Performance Liquid Chromatography with Fluorometric Detecton

Abstract

Vitamin K_3 in foods derived from livestock was measured by a method based on high-performance liquid chromatography (HPLS) combined with coulometric reduction and fluorometric detection. The vitamin extracted from foods was pirified with a Sep-Pak silica cartridge and/or a Sep-Pak silica cartridge followed by thin-layer chromatography, respectively, and then measured by using an HPLC system. The detection limit of vitamin K_3 was 50 pg/g or ml in foods.