抄録
New biochemical processes for production of vitamins and coenzymes were developed with microorganisms selected by screening. Menaquinone-4-producing process was established with derivation of mutant from menaquinone-producing Flavobacterium sp., optimization of cultural conditions, and extracellular accumulation by detergent-supplemented culture. Intracellular content of α-tocopherol of Euglena gracilis was increased by mutagenization and optimization of cultural conditions. A new system of ATP production involving the oxidative phosphorylation, oxidation of methanol and salvage synthesis of adenine nucleotide was constructed with cells of a methylotrophic yeast. A high intracellular content of cytochrome c was achieved with a cyanide-resistant mutant of an obligate methylotrophic bacterium.
