抄録
P^<32>-labeled FMN was prepared from the liver of rabbit, loaded with P^<32>-phosphate by using an ion exchange resin, IRA-400. The content of the produced FAD was determined by employing P^<32>-labeled FMN and ATP as substrates and homogenized liver of rat as enzyme, observing for 30 minutes at pH 7.4 and 35℃. The content of P^<32> per one mol was the same in FMN and FAD. Therefore, FAD is proved to have been synthesized from FMN in the following formula : FMN-P^<32> + ATP→FAD-P^<32> + Pyro. P
