Expression of GAD65, an isoform of glutamate decarboxylase, was examined on peripheral blood mononuclear leukocyte (PBMC) and on three kinds of mononuclear leukocyte cell lines, Jurkat, Ramos and HL-60. RT-PCR was performed for mRNA isolated from each cells with the probes specific to GAD65. PBMC and mononuclear leukocyte cell lines were found to give an mRNA corresponding to the full length of GAD65. Western blot analysis was carried out for each cell extracts by using GAD specific antibodies. Protein band at 80 kDa was found for PBMC that was different from 60 kDa found for each cell lines, where those protein bands were recognized by different antibodies. Results show that PBMC and mononuclear leukocyte cell lines express full length mRNA of GAD65 and GAD65 of mononuclear leukocytes differ in pattern of expression.
