Determination of Chlorogenic Acid in Rat Plasma by High Performance Chromatography after Peritoneal Administration of Compound Daqingye Injection

抄録

  A simple and sensitive high performance liquid chromatographic method has been developed for the determination of chlorogenic acid (3-O-caffeoyl-D-quinic acid) in rat plasma and applied to its pharmacokinetic study in rats after peritoneal administration of compound Daqingye injection. Plasma samples are extracted with perchloric acid. HPLC analysis of the chlorogenic acid is performed on a C₁₈ reversed-phase column using methanol-water (80: 20, v/v, pH 2.8) as mobile phase with UV detector set at 327 nm. The standard curves are linear in the range of 0.200—10.0 μg/ml (r=0.9982). The inter- and intra-day precision (relative standard deviation) was less than 9% and the accuracy (relative error) was less than 10%. The limit of quantitation was 0.200 μg/ml. The plasma concentration of chlorogenic acid shows a C_max of 7.53±0.52 μg/ml at 13.33±4.00 min with a t_1/2 of 59.10±5.42 min.