Inhibition of <small>D</small>-Amino Acid Oxidase Activity by Antipsychotic Drugs Evaluated by a Fluorometric Assay Using <small>D</small>-Kynurenine as Substrate

Sumiko IWASA; Hiroshi TABARA; Ziyu SONG; Moe NAKABAYASHI; Yuusaku YOKOYAMA; Takeshi FUKUSHIMA

doi:10.1248/yakushi.131.1111

Notes

Inhibition of D-Amino Acid Oxidase Activity by Antipsychotic Drugs Evaluated by a Fluorometric Assay Using D-Kynurenine as Substrate

Sumiko IWASA ^†, Hiroshi TABARA ^†, Ziyu SONG ^†, Moe NAKABAYASHI ^†, Yuusaku YOKOYAMA ^†, Takeshi FUKUSHIMA ^†

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Keywords: D-amino acid oxidase, antipsychotic drug, fluorescence, D-kynurenine

JOURNAL FREE ACCESS

2011 Volume 131 Issue 7 Pages 1111-1116

DOI https://doi.org/10.1248/yakushi.131.1111

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Abstract

A facile fluorometric assay using D-kynurenine as a substrate was utilized for evaluating the inhibition of D-amino acid oxidase (DAAO), which is one of the products of a susceptibility gene for schizophrenia, by commercial antipsychotic drugs, namely, chlorpromazine (CPZ), carbamazepine, sulpiride, quetiapine, and imipramine. CPZ inhibited DAAO (65.8 ± 13.2 μM, n = 3) as reported previously, and other drugs also inhibited DAAO activity. Among these, quetiapine had the smallest IC₅₀ value (19.5 ± 2.60 μM, n = 3). The proposed assay can be useful for the evaluation or screening of DAAO-inhibitory drugs.

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