Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
猪又 明子小縣 昭夫多田 幸恵長澤 明道湯澤 勝廣安藤 弘久保 喜一高橋 博海鉾 藤文田中 和良中嶋 順一鈴木 淳子植村 望美守安 貴子渡辺 大介石原 恵宇佐美 孝亀井 学河野 安昭
ジャーナル フリー

2017 年 137 巻 8 号 p. 1005-1015


 We developed a new inhalation exposure method to evaluate effects of synthetic cannabimimetics that are being distributed as new, unregulated drugs in the Tokyo area. We selected the commercial product “SOUTOU” containing AB-CHMINACA and 5F-AMB as the test drug and dried marshmallow (Althaea officinalis) leaves as the negative control. A half cigarette packed with dried marshmallow leaves or SOUTOU was ignited, then mainstream smoke from each was delivered to five mice in an exposure box. After the cigarettes were fully consumed, neurobehavioral observations and a catalepsy test were performed at 15, 30 and 60 min after exposure. The effluent air from the exposure box was poured into impingers containing acetonitrile (first impinger) and dimethyl sulfoxide (second impinger). The resulting solutions were analyzed to assess decomposition of the synthetic cannabimimetics. Mice exposed to SOUTOU smoke showed many excitement behaviors and some suppressive behaviors at 15, 30 and 60 min. These clearly included cannabimimetic specific pharmacological actions. Negative control mice also showed some suppressive behaviors at 15 min but these were attenuated at later times, nearly disappearing at 60 min. In addition, the behavioral effects observed in controls were less pronounced than those in SOUTOU exposed mice. The inhalation exposure method developed in our study would be effective for determining cannabinoid specific pharmacological effects of illegal drugs, as well as for assessing the presence of active compound(s) by comparing the test substance with a negative control.

© 2017 The Pharmaceutical Society of Japan
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