尿中エストロゲンの免疫学的測定法の研究(第1報)抗体の作製とその免疫学的性質およびラジオイムノアッセイ

抄録

Estriol antisera were raised and studied for the use in radioimmunoassay (RIA) of urinary estrogen as an aid of diagnosis of feto-placental function. Antisera to estriol-related six haptens, i.e., estriol 16-glucuronide (E₃-16-G), estriol 16-hemisuccinate (E₃-16-succ), estriol 17-hemisuccinate (E₃-17-succ), estriol 16, 17-dihemisuccinate (E₃-16, 17-succ), estriol 3-carboxymethylether (E₃-3-CME) and estriol 3-glucuronide (E₃-3-G), were obtained by immunizing rabbits with the respective haptenbovine serum albumin (BSA) conjugate. Antiserum to E₃-16-G-BSA reacted significantly with E₃-16-G, C-17 conjugated estrogens and free estrogens. But it did not react with C-3 conjugated estrogens and other steroids. Similar properties were observed with antisera to E₃-16-succ-BSA, E₃-17-succ-BSA and E₃-16, 17-succ-BSA, but their reactivities with C-16 or C-17 conjugated estrogens were slightly lower than that of anti-E₃-16-G-BSA serum. Among antisera which were raised against estriol linked to BSA via hemisuccinate varying in linking posirion, anti-E₃-16-succ-BSA serum highly reacted with E₃-16-G, while anti-E₃-17-succ-BSA did with E₃-17-G. Anti-E₃-16, 17-succ-BSA serum reacted with C-16 and C-17 conjugated estrogens to nearly the same extent. Antisera to both E₃-3-CME-BSA and E₃-3-G-BSA reacted with estrogens conjugated at C-3 position, but failed to react with C-16 or C-17 conjugated estrogens. The latter antiserum reacted more specifically with E₃ and E₃ 3-G than the former. Estrogen values in pregnancy urine samples estimated by the RIA by use of anti-E₃-16-G serum were not influenced by other components in urine, and showed a good correlation of y=0.81x-1.28, r=0.983 with those determined by the currently available colorimetric method with E₃-kit.