Abstract
The determination of tropane alkaloids (atropine and scopolamine), contained in gastrointestinal drugs and scopolia roots, was studied using a gas chromatograph equipped with a flame thermoionic detector with specific sensitivity to nitrogenous compounds. Dexil 300 GC, Silicone OV-17 and Thermon 1000 were used for the stationary phase. Homatropine to Dexil 300 GC and Silicone OV-17, and diphenhydramine to Thermon 1000 gave good results as an internal standard substance. Between the peak height ratio of alkaloids to the internal standard substance and the alkaloid contents, a linear response at the range 25 to 75 ng atropine and 2.5 to 7.5 ng scopolamine was recognized. The determination of atropine and scopolamine in gastrointestinal drugs and scopolia roots was easily carried out by this method without any interference.
