ラクト-N-テトラオース誘導体の¹³C-Nuclear Magnetic Resonanceスペクトル

抄録

The completely proton-decoupled ¹³C-nuclear magnetic resonance spectrum of the 1, 6-anhydro derivative of O-β-D-galactopyranosyl-(1→3)-O-2-acetamido-2-deoxy-β-D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→4)-D-glucopyranose (lacto-N-tetraose) (8) was measured in D₂O. The signals were assigned by direct comparison of the spectra with those of 1, 6-anhydro-β-lactose (lactosan) (5) and O-β-D-galactopyranosyl-(1→3)-O-2-acetamido-2-deoxy-β-D-glucopyranose (lacto-N-biose I) (7), and also by examination of the selectively proton-decoupled, and the partially relaxed Fourier transform (PRFT) spectra. The effect of the glycosylation shift was discussed, and the shift values calculated served for the confirmation of the structure of 8.