1981 Volume 101 Issue 1 Pages 72-78
Chromogenic method was examined for simple and quick determination of the activities of amylases in gastrointestinal drugs. The synthetic chromogenic substrates from Cibachron Blue 3GA, various amyloses and starches were employed in this experiment. Commercial chromogenic substrate, Amylochrome Roche, had the most superior reactivity to the synthetic Cibachron Blue 3GA-amyloses or starches. α-Amylase, endo type glucan hydrolase, had very high activity, but β-amylase and amyloglucosidase, exo type glucan hydrolase, had no activity towards the chromogenic substrates. So it suggested that this method was α-amylase specific. The activity of amylase in gastrointestinal drugs was determined simply and quickly by this method. It can be useful for the routine quality control of the gastrointestinal drugs.
