Abstract
The method for the analysis of binding parameter fitting for drug-protein interaction by use of a pocket programmable calculator from curvilinear Scatchard plot in the case of two independent sets of the binding sites is described. The comparison of this analysis with a non-linear regression analysis by least square method by using a digital computer showed that the calculator solution is as rigorous as and/or more flexible than the computer solution especially for the fitness of the scattered binding data and also much more accessible. The method is applied to estimate the binding parameters for the practical binding data of caprylic acid and tolbutamide with serum albumin obtained from the radiostereo assay.
