High-performance liquid chromatographic procedure for the determination of ascorbic acid (AsA) and flavin adenine dinucleotide (FAD) in intravenous fluids was developed and applied to observe the stability and interactions in clinically prepared intravenous fluids. This method provided a simple and rapid technique for the determination of AsA and FAD in intravenous admixtures. A significant decomposition with respect to AsA in multiple electrolyte solution was noted, while no significant decomposition of AsA and FAD in intravenous hyperalimentation fluids was noted for a 6 hour period of the study. Non-stabilizer containing such solution as SOLITA-T3 tends to accelerate AsA decomposition. The change in such environmental factors as stabilizer, pH, dissolved oxgen, glucose content, catalyzer and light could leads to these different results.
