高速液体クロマトグラフィーによるローヤルゼリー中の10-ヒドロキシ-2-デセン酸の定量

抄録

A method for the quantitative determination of 10-hydroxy-2-decenoic acid (10-HDA) in royal jelly was establishd by high-performance liquid chromatography (HPLC) by use of a reversed-phase stationary phase. The 10-HDA extracted with CH₂Cl₂ from royal jelly was determined by HPLC under the following operating conditions : column, LiChrosorb RP-18 ; mobile phase, 55% methanol in water (pH 2.5) ; monitoring, ultraviolet absorption at 205 nm : internal standard, suberic acid ; temperature, 50°C. This method was simpler and more rapid than gas chromatographic method. It was successfully applied to the determination of 10-HDA contents in raw royal jelly, granules and tablets containing royal jelly.