1982 Volume 102 Issue 7 Pages 670-677
Tissue homogenate could react with thiobarbituric acid (TBA) and gave the maximum coloration at around pH 2.5, even if the peroxidation of the tissue is accelerated by aging, CCl4- or hexachlorobenzene (HCB)-intoxication. Isolated methyl linoleate hydroperoxide (MLHPO) showed the optimum pH at 3.0-4.0 when reacted with TBA in the presence of an inorganic- or heme-iron catalyst, whereas the optimum pH shifted to 2.5 or so when the reaction was catalyzed by defatted homogenate or iron with albumin. Addition of sodium dodecyl sulfate (SDS), which is sometimes employed as a stimulator of TBA reaction, brought about rather diverse effects in the coloration of MLHPO and tissue homogenate. The TBA value of isolated MLHPO was enhanced by the addition of SDS and the optimum pH was 3.5 irrespective of the catalysts. By the addition of SDS into tissue homogenate the maximum coloration could be obtained at pH 1.0 in all cases (young, aging, CCl4- and HCB-intoxication). Moreover, the difference of TBA value due to aging or intoxicating became insignificant.
