海洋細菌Vibrio anguillarumの培養〓液物質の抗腫瘍作用とマウス免疫機能への影響

抄録

Antitumor activity of culture filtrate substance (CFS ; MW≨13000) isolated from three strains (TA-3, H-81, and PB-1) of Vibrio anguillarum, a marine bacterium, against Ehrlich carcinoma cells in ddY mice was investigated. Sixty days after intraperitoneal (i.p.) inoculation of 1×10⁵ tumor cells, groups of mice injected i.p. CFS (250μg/d/mouse×6 times) survived 20-80% and their mean survival was 40-55 d against the mean survival of 24 d in the control group. When 1×10⁶ tumor cells were inoculated subcutaneously, CFS injected intraveously caused the inhibition of the tumor-growth. CFS of PB-1 strain exhibited markedly an adjuvant effect on antibody response in vivo against sheep erythrocytes. CFS of TA-3 strain was capable of increasing the incorporation of ³H-thymidine into in vitro cultured spleen cells of C57BL/6 mice, but both CFS of H-81 and PB-1 do not increase the incorporation.