Abstract
A method was developed for the determination of taurine in whole blood and plasma by gas-liquid chromatography. The method involves a deproteinization, conversion of taurine into its N-isobutyloxycarbonyl di-n-butylamide derivative and chromatography on a 5.0% SE-54 column with hydrogen flame ionization detection. 3-Amino-1-propanesulfonic acid was used as an internal standard. The average recoveries of taurine added to whole blood and plasma samples were 97.6 and 98.3%, respectively. The mean values of taurine content in whole blood and plasma of thirty normal subjects were 163.9±35.8 and 45.3±7.4 nmol/ml, respectively.
