Effects of Cyclic Adenosine 3', 5'-Monophosphate and Calcium on Cellular Function and Growth of Mast Cells

Abstract

In many mammalian cells, cyclic adenosine 3', 5'-monophosphate (cAMP) and calcium (Ca²⁺) served interrelated second messenger functions in the control of cellular function and growth. We have investigated; (i) the effects of cAMP and Ca²⁺ on the control of histamine release from mast cells stimulated by active oligomers, of which releases are dependent on extracellular Ca²⁺ or not. (ii) the fluctuations of cAMP levels, PGE₁-binding, and several cellular functions because of the cells respond to Bt₂cAMP, and PGE₁ with decreased growth rate and increased expression of more mature, differentiated mast cell phenotypes, and also because PGE₁ specifically binds to the cells and elevates cAMP levels. (iii) the cell cycle-dependent changes of cAMP and Ca²⁺ levels, and certain cellular functions in synchronous mastocytoma P-815 cells.