障害赤血球に対するアデノシン三リン酸-塩化マグネシウム(ATP-MgCl₂)の作用

抄録

The present study was undertaken to determine if adenosine triphosphate-magnesium chloride (ATP-MgCl₂) would have beneficial effects on the membrane electrolytes transport and deformability of shocked red blood cells (RBC) with impaired cellular energy metabolism. Shocked RBC suspensions were obtained by incubation of 10% rabbit RBC suspension in phosphate buffered saline at 37°C for 14 h. Cellular ATP level was reduced to approximately 6% of normal value by this incubation. ATP-MgCl₂ (100 μM), added to the incubation medium along with glucose (10 mM) after 14 h of incubation, significantly prevented further decreases in cellular ATP and 2, 3-diphosphoglycerate (2, 3-DPG) levels. Increases in intracellular sodium concentration and potassium leakage from RBC were counteracted by simultaneous ATP-MgCl₂ and glucose treatment and pH of the incubation medium showed significant reduction compared with the control. It was also observed that glucose utility and deformability of shocked RBC were improved by ATP-MgCl₂ and glucose treatment. These results indicate that ATP-MgCl₂ would be helpful to restore the impaired RBC function by improving the intracellular energy metabolism.