サイズ排除高速液体クロマトグラフィー (SEC) によるセラペプターゼの定量 (第1報)

抄録

Enzymatic assay, by use of casein as substrate, has long been used for the quantitative determination of serrapeptase. The method, however, either requires a complicated operation or results with poor reproducibility and low sensitivity. Therefore, we tried to investigate a method for the determination of serrapeptase by using high performance steric exclusion chromatography (SEC). An organic microparticulate stationary phase (diol bonded silica gel), an aqueous mobile phase and ultraviolet detection at 278 nm were adopted. Consequently, we found a linear relationship between SEC method and conventional enzymatic assay. Simultaneously, the molecular weight of serrapeptase was estimated from calibration curve of SEC. As the result, the new SEC method may be superior to the alternate techniques for the determination of serrapeptase.