Abstract
The metabolic reduction of acetohexamide was examined by using the rat liver and kidney. This reduction activity was observed in both the microsomal and cytosolic fractions of these tissues. The Km and Vmax values for the reduction activity in the microsomal fractions of the liver and kidney were higher than those in the cytosolic fractions. The optimum pH for acetohexamide reduction activity in the microsomal and cytosolic fractions of the liver were 4.5 and 5.0, respectively, but those of the kidney ranged from 6.0 to 7.0 Furthermore, the sensitivities to various inhibitors were different among the microsomal and cytosolic fractions of the liver and kidney. These findings indicate the heterogeneity of acetohexamide reducing enzymes in the microsomal and cytosolic fractions of the rat liver and kidney.
