1990 Volume 110 Issue 2 Pages 155-158
This paper describes the preparation of 7-dimethylaminocoumarin-3-isocyanate (MACI) having 7-dimethylaminocoumarin as a high sensitive fluorophore together with an isocyanate group as a reacting moiety and a preliminary investigation as a precolumn flourescence derivatization reagent for alcohols in high performance liquid chromatography (HPLC). Alcohols were derivatized quantitatively into the corresponding fluorescent urethanes by treating with MACI at 80°C for 10 min. The derivatives were subjected to HPLC on an Inertsil ODS (250×4.6 mm i.d., 5 μm) column with acetonitrile-ethanol-water (50 : 49 : 1) as mobile phase and monitored with an excitation wavelength of 382 nm and an emission wavelength of 479 nm. The detection limit of cholestanol was 10 fmol for an injection volume of 10 μl.
