コブラ(Naja naja kaouthia LESSON)の薬理学的研究(第3報)コブラの肝臓及び胆嚢の50%エタノール抽出エキスのマウス網内系の貪食能に及ぼす影響

抄録

Effects of 50% ethanolic extracts from the liver and the gall bladder of Naja naja kaouthia LESSON (COB-L or COB-G) were studied on the phagocytic activity of a mouse reticuloendothelial system. The clearancerate of carbon was shortened by the oral administration of COB-L or COB-G (50 or 200 mg/kg). COB-L or COB-G promoted the phagocytosis of latex by peritoneal macrophages (Mφ). Furthermore, effects of these extracts on the peritoneal Mφ were biochemically investigated using in vitro experimental models. The activities of two lysosomal enzymes, acid phosphatase and β-glucuronidase, and that of lactate dehydrogenase in the peritoneal Mφ were enhanced when the Mφ were cultured with COB-L or COB-G (10-100 μg/ml) for 4 d. In addition, the consumption of glucose in the culture media by the Mφ was also enhanced by culturing the media with COB-L or COB-G. When COB-L and COB-G were given orally immediately before and 16 h after the application of picryl chloride (PC) or sheep red blood cell (SRBC), these extracts at a dose of 200 mg/kg did not show any inhibitory effects on the swelling by picryl chloride-inducing contact dermatitis (PC-CD) and by sheep red blood cell delayed type hypersensitivity (SRBC-DTH) in mice. However, these extracts inhibited the immunosupression with the SRBC 1×10⁹ cells priming and with the freezed and dried ascites of Ehrlich carcinoma-bearing mice containing immunosupressive substances (EC-sup). These results suggest that COB-L or COB-G biochemically activates the mouse peritoneal Mφ, and promotes the phagocytic activity of the Mφ and the interaction between Mφ and T cell.