Preparation of Water-Soluble Hydronaphthoquinone Derivatives and Its Application to the Determination of Hydrogenperoxide in Clinical Chemistry

Abstract

Several 1-alkoxy-2, 3-dichloro-4-hydroxynaphthalene derivatives were prepared. These compounds were oxidized by hydrogen peroxide in the presence of a peroxidase to give 2, 3-dichloro-1, 4-naphthoquinone, which then reacted with various alkyl benzoylacetates to develop blue coloration with absorption maximum at >600 nm. The color development reaction can be applied to the determination of appropriate serum constituents such as cholesterol.