澱粉糖の不醗酵成分について (第2報)

葡萄糖から生成せる6-α-glucosylglucose及びgentiobioseの定量法について

抄録

Gentiobiose was quantitatively determined by the increase of its reductive power by the hydrolysis of gentiobiose by Takadiastase. Gentiobiose was determined in a similar manner in the non-fermenting sugars formed from glucose. By the use of Somogyi's microanalytical method for sugar determination, only about 2mg. of sample is necessary. Following observations were also obtained:
1) The reductive power of gentiobiose against Somogyi's reagent corresponds to 0.542 of glucose.
2) The hydrolysis rate of commercial Takadiastase against gentiobiose is about 10 times faster than the hydrolysis rate of the standard emulsin prepared from almond seeds by the Helferich method (omitting purification by silver oxide).