Studies on the Salivary Gland Hormones. XIII

Purification of Parotin, the pH 5.4-Precipitate from Bovine Parotic Glands by Fractional Precipitation with Sodium Sulfate

抄録

The pH 5, 4-precipitate extracted from bovine parotic gland and possessing the action of lowering the calcium level in rabbit serum was subjected to fractional precipitation with sodium sulfate. The fraction possessing the most potent action was extracted with physiological saline solution by which a preparation was obtained possessing ten times the strength of the crude material. The total potency of various fractions obtained by fractionation was about 80% of the total potency of the crude material. Moisttire content of various preparations were estimated at the time of potency tests by which it was shown that the potency was greatly decreased when the drying process of the preparation was poor. The moisture content of the preparation must be below 7%, the most ideal being around 5%. By the extraction with saline solution of acetone. dried pH 5.4-precipite, a certain amount of increase in potency was effected.