植物成分の微量化学的研究 (第17報)
糖及びフラボノイドの濾紙電気泳動法
1953 年 73 巻 10 号 p. 1095-1097
詳細
1953 年 73 巻 10 号 p. 1095-1097
Compounds which possess hydroxyl groups in a vicinal position undergo change in their electrical conductivity in boric acid solution by forming some kind of a complex salt of boric acid. Based on this fact, sugars and flavonoids can be separated by paper electromigration using boric acid or borax solution as the electrolyte. Sugars which could not be separated by paper partition chromatography could be separated easily by this method. In the case of flavonoids, the distance of migration was found to be proportional to the number of the vicinal hydroxyl groups in their molecule. Coloring agent used for sugars was aniline biphthalate, and lithium aluminum hydride was found to be the most sensible color reagent for flavonoids.
