抄録
By heating dibromofluorescein and mercury acetate, in 1:4 molar ratio, at pH 4-5 for 6 hours, a mercury compound giving analytical values of Hg 37.3% and Br 19.67% is obtained when the reaction medium was around pH 4, and of Hg 34.7% and Br 20.8% when around pH 5, for which formulae (VII) and (VIII) were respectively assumed. When these compounds were made soluble by the application of a calculated amount of sodium hydroxide solution, a mixture of the sodium salts of di-(V) and monohydroxymercuri (VI) compounds was formed. These assumption were drawn from the absorption spectra and confirmed by the following facts:
1) Application of iodine solution and derivation of HgOH to -I followed by ace-tylation resulted in the separation of two kinds of acetates of dibromodiiodo- and dibromomonoiodo-fluorescein.
2) Paper chromatography developed with 3% ammonia water gave two spots of Rfca. 0.07 and 0.38.
3) Separation by column chromatography through activated magnesium oxide gave a dimercury and monomercury compounds.
It has been found that the so-callced Mercurochrome is not the sodium salt of mo-nohydroxymercuridibromofluorescein alone but always contains the dimercury compound and a small amount of unreacted dibromofluorescein, by examination with paper chro-matography.
