抄録
Based on the paper chromatographic analysis of protamine zinc insuline carried out by Robinson, determination of purity of crude insuline powder was attempted. It was found that the insuline band (Rf 0.47) and the crude protein band (Rf 0) on such a chromatogram were separated, as in the Robinson method, but a substance appeared at Rf 0.40. This was assumed to be an insuline-like substance but not possessing potency equal to that of insuline. Determination of insuline through the combination of this chromatogram elution and photoelectrocolorimetry was found to give results with mean error of within 1 UV/mg. with that of bioassay.
