The content of chlorophyll in some commercial toothpastes containing chlorophyll was determined using a quantitative bridge. A quantitative bridge of 2mm.-width and 60mm. length was made at a position 60mm. from the starting line and the paper was developed at 25° with a mixture of butanol: ethanol: 1% ammonium chloride solution (2:1:1). Calibration curves shown in Figs. 1-3 were obtained. The accuracy by this method was within 4.3-4.6% and better results compared to the existing method were obtained.