Abstract
Paper chromatography of the glycoside A from Engelhardtia formosana with 20% methanol as the developing solvent gives two spots (cf. Table I). Isolation of the two substances was attempted by the chromatopile method by which they were separated into engelitin (I) and kaempferol 3-L-rhamnoside (II) as yellow plates, m.p. 172-174°, C21H20O10⋅2H2O, which agreed with the properties of afzelin except for the water of crystallization. Recrystallization of a mixture of (I) and (II) afforded a substance completely identical with the glycoside A, which was thereby confirmed as a mixed crystal.
