麦芽根飼料より分離せる有害物質産生糸状菌の研究 (第6報)
Patulinの微量検出法と定量法
1956 年 76 巻 12 号 p. 1375-1377
詳細
1956 年 76 巻 12 号 p. 1375-1377
It was found that patulin colored orange yellow by reacting with phenylhydrazine and reddish brown by reacting with alkalis. Microdetection of this substance by paper chromatography, utilizing these reactions, and a method of its determination by photocolorimetry were established. Microdetermination by the use of antibacterial activity of patulin was devised by the cup method using Staphylococcus aureus. These methods are helpful in the microdetection or -determination of patulin from culture medium or wheat germs on which molds have grown. The limit of detection is 1-2γ, while the range of determination is 4-16γ/cc by the phenylhydrazine process, 0.2-0.8mg/cc with Staph. aureus.
