1956 Volume 76 Issue 2 Pages 172-175
A possible use of ion exchange resins for isolation of Aureomycin in urine was investigated. It was found that Aureomycin and components of urine were retained on strong cation exchangers, Amberlite IR-112, but it seemed possible to remove urine materials by the following procedures. Aureomycin can be eluated with 1N HCl-EtOH solution (8%) while the use of 1.5N AcOH-EtOH or 0.2N HCl-EtOH eluates the components of urine alone, not Aureomycin. A colorimetric determination of Aureomycin present in the eluate involves reaction of this antibiotic with boric and sulfuric acid mixtures, as previously described.