Studies on Submaxillary Gland. IX.

Chemical Properties of S-Parotin and Purification of Serum P Lever Decreasing Principle from Bovine Submaxillary Gland

Abstract

Sixteen kinds of amino acids were detected as the componental amino acids in S-parotin of 96.1% purity by electrophoretic test. The terminal amino acids in S-parotin were found, by the DNP method, to be alanine, histidine, and arginine. DNP-S-parotin did not have the potency of decreasing serum calcium level in rabbits while the elementary analysis of S-parotin gave the values of 49.53% carbon, 7.35% hydrogen, and 14.05% nitrogen. The fractionation of the principle which decreases the serum phosphorus level was obtained with 71.9% purity, which contained 9.49% nitrogen, and its isoelectric point was pH 4.0. The effect of S-parotin on the amount of pyruvic acid in the blood was similar to that of parotin but the principle which decreases serum phosphorus level effected marked decrease of pyruvic acid in the blood 30 minutes after its injection, the amount of the acid returning to the normal after 6 hours.