Studies on the Constituents of Coix Species. IV
Paper Partition Chromatography of Coixol
1956 Volume 76 Issue 9 Pages 1077-1078
Details
1956 Volume 76 Issue 9 Pages 1077-1078
Paper partition chromatography of coixol by the one-dimensional ascending method using butanol saturated with 27% ammonia water gives a spot at Rf 0.63. Fuming nitric acid was sprayed as the coloring agent. The ether extract of various portions of Coix Lachryma-Jobi L. and C. Lachryma-Jobi L. var. frumentacea MAKINO was submitted to paper partition chromatography, the portion corresponding to coixol was cut out, and extracted with ethanol. Colorimetric determination was carried out with such an extract at the characteristic wave length (291mμ.) of coixol. The amount of coixol was the largest in the root of both plants, a small amount was found in the stem near the root, but only a trace in the upper portion of the stem. Coixol was entirely absent in the fruit and leaves. Although 40 genera and 50 species of Graminae plants were examined, coixol was found in none but the Coix genus.
