Ag-RNA-Complexを介する核酸の精製法について (第3報)

抄録

The fact that the ribonucleic acid regenerated from its silver complex is of higher purity than that of commercial yeast nucleic acid preparation suggests the possibility of the use of this process for purification of ribonucleic acid preparations. The commercial preparation of sodium yeast-ribonucleate was found by ninhydrin reaction tests to be contaminated with a fair amount of proteinic substances. Therefore, such a commercial preparation was submitted to comparative tests of deproteinization by purification through silver-ribonucleic acid complex formation and by Sevag's chloroform gel method. The former was found to be highly effective, the deproteinization effect obtained finally by nine repetition of the chloroform gel process being attained in one purification through the silver-ribonucleic acid complex.
It was assumed that the deproteinization of this purification through the silver complex is effected by colloidal absorption of occluded impurities by the silver sulfide formed. Therefore, copper nitrate or nickel nitrate and hydrogen sulfide can also effect purification of nucleic acid but the yield in such a case is only around 20-40% against that of 80-90% by the silver complex method. Further, the purification process becomes more complicated by the use of copper or nickel salts.